e-journal
Responses in gas exchange and water status between drought-tolerant and -susceptible soybean genotypes with ABA application
The purpose of this studywas to investigate the physiological responses of drought-tolerant and drought-susceptible soybean genotypes to exogenous abscisic acid (ABA) application during progressive soil drying at seedling stages. Five-day old soybean seedlings were transplanted into PVC tubes filledwith soilmixture. Seedlingswerewatered dailywith similarwater volumes until second trifoliate leaves emerged, and thereafter soil drying with or without exogenous ABA
applicationwas imposed. Half of the seedlings of each genotype were left for regularwatering as control plants. Soil water status declined significantly over seven days of withholding water supply for both genotypes. Leaf expansion rate, stomatal conductance (gs), leaf water potential (ψw), and relative water content of leaves (%RWC) declined significantly under soil drying as well as soil drying with ABA application, compared to their values for well-watered soybean genotypes. However, a drought-tolerant genotype (C12) responded more rapidly than a drought-susceptible genotype (C08) after imposition of soil drying and soil drying with exogenous ABA. In addition, application of exogenous ABA to water-restricted soybeans resulted in higher %RWC and ψw in the drought-tolerant than in the drought-susceptible genotype. Compared to the drought-susceptible genotype, the drought-tolerant genotype was more responsive to exogenous ABA application, resulting in a higher root-to-shoot ratio.
Keywords: Drought stress Exogenous ABA Leaf relative water content Stomatal conductance Soybean genotypes
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