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Enhanced immune response and resistance to white tail disease inchitin-diet fed freshwater prawn, Macrobrachium rosenbergii
Chitin is one of the natural biopolymer found abundantly in the shells of crustaceans, insects and in cellwalls of fungi. In this study, we determined the effect of dietary administration of 0.5, 0.75 and 1% chitinon the immune response and disease resistance in freshwater prawn, challenged against Macrobrachiumrosenbergii nodavirus (MrNV) and extra small virus (XSV). We observed a significantly enhanced immuneresponse, indicated as higher prophenoloxidase activity and respiratory burst of hemocytes, in 0.75%chitin-diet fed prawns compared to the chitin-free-diet fed prawns. Importantly, the relative percentsurvival (RPS) following challenge with white muscle disease (WTD) viruses was found relatively high inM. rosenbergii fed with diet containing 0.75% chitin (63.16%), suggesting an increased resistance to diseasesusceptibility. These results indicate that the incorporation of chitin in prawn diet would be beneficial instimulating the immune response and thereby developing resistance against diseases.
Keywords:ChitinProphenoloxidaseRespiratory burstRelative percent survivalMacrobrachium rosenbergiiWhite tail diseasea
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