e-journal
Stress responses in rohu, Labeo rohita transported at differentdensities
Transportation of fish, although stressful, is an inevitable procedure in aquaculture practices. In thisstudy, we investigated the changes in biochemical and haematological indices in advanced fingerlingsof rohu, Labeo rohita in order to optimize the packing density as is imperative to avoid transportationstress and associated mortality. The fish (14.00–15.00 cm) were packed in sealed double layer oxygenpacked polythene bags of 30 L capacity containing 7.5 L of water and transported for 2 h and 30 min ina motor vehicle at three packing densities, viz., 67 g L−1, 134 g L−1, and 201 g L−1and transported fromhatchery to Kolkata, India covering a distance of about 55 km. A cumulative mortality of 30% was recordedduring and after transportation in fish transported at 201 g L−1; while the other two packing densitiesrecorded no mortalities. Transportation and packing density induced stress affected the functioning ofliver and kidney. The activities of transaminases and lactate dehydrogenase, breakdown of protein andcreatinine phosphate, cortisol induced glucose production were significantly increased in advanced rohufingerlings to cope with the energy demand posed by transportation and higher packing density stress.Low levels of dissolved oxygen and high levels of ammonia-nitrogen were noticed in different packingdensities after transportation. The results suggested that the optimum packing density for advanced rohufingerlings for 2–3 h duration of transport would be 134 g L−1, which kept the stress at low levels. Theserum alanine aminotransferase levels can serve as a good marker for fish liver health as the incrementwas more prominent when subjected to transportation stress.
Keywords:RohuShort-term transportationBiochemical indicesHaematological indicesa
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