e-journal

Molecular characterization and expression analysis of interferon (IFN-) gene in Labeo rohita (Ham.)

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Genes coding for interferon  (IFN-) has been cloned and studied for functional analysis in many species.Labeo rohita commonly known as rohu, is a commercially important and widely cultured fish in South-East Asia. IFN- of rohu was amplified with primers based on conserved regions of other related species.5and 3end were amplified using RACE PCR. The PCR products were cloned and sequenced. The sequenceanalysis of IFN- revealed that the genomic sequence consists of 1803 bp with 3 introns and 4 exons. AnORF of 552 nucleotides encodes for a protein of 183 amino acids. Matured IFN- protein has 157 aminoacids with a signal peptide of 26 amino acids. Mature protein has a molecular weight of 18.7 kDa. FourRNA instability regions (ATTA) and one polyadenylation signal (AATAAA) were seen in 3UTR region. IFN- signature sequence, one glycosylation site and nuclear localisation region were also observed. Intronsplicing motif (ag/gt) was observed in genomic DNA. The gene sequence shows high similarity with thatof Cyprinus carpio. The expression analysis for IFN- in kidney, spleen, liver and gill was done by RealTime PCR. Expression of IFN- was more in spleen compared to gill, liver and kidney. Cultured kidneycells were induced with poly I:C and samples were collected at 2, 8, 24 and 48 h post-induction to studythe effect on poly I:C of expression of IFN-. IFN- expression increased 30 fold at 2 h post- inductionand increased further till 24 h.

2015Keywords:Interferon CharacterizationExpression analysisa

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Informasi Detail

Judul Seri

Aquaculture Reports

No. Panggil

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Penerbit

: Elsevier., 2015

Deskripsi Fisik

Aquaculture Reports 2 (2015) 97–105

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

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