e-journal
Secondary stress responses of zebrafish to different pH: Evaluation ina seasonal manner
Stress is one of the most critical factors in fish health. The response to stress in fish is characterized bythe stimulation of the hypothalamus, which results in the activation of the neuroendocrine system anda subsequent cascade of metabolic and physiological changes. The present paper deals with the stressresponses of water pH on certain biochemical indices and hematological parameters of zebrafish in aseasonal basis. Zebrafish were exposed to different pH e.g., 7.2, 5.0 and 10.0 and the secondary stressresponses were observed. The result showed that exposure to pH 5.0 and 10.0 on zebrafish exertedstresses with reference to seasons. Higher values of blood glucose content observed in the month ofsummer than the month of winter and females showed higher values than males. Chronic effects of pHon the hematological parameters were also significant between these seasons. In conclusion, exposureto pH at sub-lethal concentrations induced biochemical and hematological alterations in zebrafish andoffers a simple tool to evaluate the potential risk of polluted water (acid and base) to fish.
Keywords:Blood glucoseHematological parameterspHStressSecondary stress responsesZebrafisha
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