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Growth rate analysis and protein identification of Kappaphycusalvarezii (Rhodophyta, Gigartinales) under pH induced stress culture

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Environmental pH is one of the factors contributing to abiotic stress which in turn influences the growthand development of macroalgae. This study was conducted in order to assess the growth and physio-logical changes in Kappaphycus alvarezii under different pH conditions: pHs 6, ∼8.4 (control) and 9. K.alvarezii explants exhibited a difference in the daily growth rate (DGR) among the different pH treat-ments (p ≤ 0.05). The highest DGR was observed in control culture with pH ∼8.4 followed by alkaline (pH9) and acidic (pH 6) induced stress cultures. Protein expression profile was generated from different pHinduced K. alvarezii cultures using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by protein identification and analysis using matrix-assisted laser desorption/ionizationtime-of-flight mass spectrometer (MALDI-TOF-MS) and Mascot software. Ribulose bisphosphate car-boxylase (Rubisco) large chain was identified to be up-regulated under acidic (pH 6) condition during thesecond and fourth week of culture. The findings indicated that Rubisco can be employed as a biomarkerfor pH induced abiotic stress. Further study on the association between the expression levels of Rubiscolarge chain and their underlying mechanisms under pH stress conditions is recommended.

Keywords:Growth rateKappaphycus alvareziipHProtein expressionRed algaea

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Informasi Detail

Judul Seri

Aquaculture Reports

No. Panggil

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Penerbit

: Elsevier., 2015

Deskripsi Fisik

Aquaculture Reports 2 (2015) 112–116

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

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