e-journal
Parental contribution and growth hormone gene polymorphismassociated with growth phenotypes of red sea bream Pagrus major inmass production: A case study
Red sea bream is one of the most important aquaculture fish species in Japan. To improve the productivityof this fish during seed production, improved growth traits and reduced size variation are needed. In thisstudy, we assessed parental contribution of fast- and slow-growing individuals observed in two differentrearing phases in a mass production lot: (1) 50 dph reared in a tank and (2) 200 dph reared in a netcage. We also assessed GH gene (pmaGH) polymorphisms based on a previously developed minisatelliteDNA marker. Specific broodstock individuals were significantly associated with fast- or slow-growingindividuals at 50 dph and 200 dph. Significant differences in pmaGH minisatellite allele frequencies wereobserved between fast- and slow-growing groups at 50 dph in the frequency of two alleles (pmaGH-740and pmaGH-900, respectively). Combining the results of DNA parentage analysis and pmaGH minisatelliteallele analysis, one dam and two sires, possessing pmaGH-740, were significantly associated with theslow-growing groups. These results suggest that the minisatellite marker of pmaGH could be a usefultool for growth selection of this fish species.
Keywords:Red sea breamGrowth hormoneGrowth traitMinisatelliteDNA parentagea
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