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Experimental induction of motile Aeromonas septicemia in channelcatfish (Ictalurus punctatus) by waterborne challenge with virulentAeromonas hydrophila

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Motile Aeromonas septicemia (MAS), caused by virulent clonal isolates of Aeromonas hydrophila (vAh), isemerging as a major disease in catfish (Ictalurus punctatus) aquaculture in the Southeastern United States.Predisposing conditions leading to vAh infection in catfish were however largely unknown. The objectiveof this study was to investigate factors that predispose catfish to vAh infection and establish a waterbornechallenge model that mimics natural occurrence of MAS. Results of this study indicated that wounding onthe fish body surface was one of the key factors that predisposed catfish to vAh infection via waterborneroute. Relatively uniform wounds were created by clipping part of the fish adipose fin. Adipose fin clipped(Af-clipped) fish behaved normally in terms of swimming and feeding and no mortality occurred in thecontrol treatment (a mock challenge). When subjected to challenge in vAh-infected water, Af-clipped fishwere highly susceptible, showing typical symptoms of MAS observed in the field. The mortality rate of Af-clipped fish was significantly associated with vAh concentration, challenge time and water temperature.About 90% mortality occurred within 48 h when Af-clipped fish were challenged for 1 h with vAh at theconcentration of 2 × 107colony forming units per mL of water (27 ± 1◦C). The waterborne challengemodel was further tested using four field isolates including A. hydrophila and A. veronii. All vAh isolatescaused about 90% mortality of Af-clipped fish and one isolate of Aeromonas veronii caused no mortalityunder the same challenge conditions. The waterborne challenge model described in this study wouldfacilitate urgently-needed studies of MAS prevention (such as wound avoidance and healing) and control(such as prophylactic vaccination; antibiotics treatment and probiotics screening).

Keywords:Adipose finChannel catfishMotile Aeromonas septicemiaVirulent Aeromonas hydrophilaWaterborne challengea

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Informasi Detail

Judul Seri

Aquaculture Reports

No. Panggil

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Penerbit

: Elsevier., 2016

Deskripsi Fisik

Aquaculture Reports 3 (2016) 18–23

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

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