e-journal

An optimized group mating design and determination of theadmixture rate in Nile tilapia families

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The Nile tilapia is the most productive and internationally traded food fish in the world. Many Niletilapia families are needed to develop breeding programs for this species. The aim of this study was todevelop Nile tilapia families using the Oreochromis species unique breeding characteristics (male territoryestablisher and female mouth brooders) and determine the mixing rate within each family. Both male andfemale fish were cultured in one pond in the reproductive season and the female mouths were checkedevery 5–8 days. When embryos were present, they were removed and cultivated independently as afamily. As a result, 45 families were developed from the 60 female fish sampled. The embryo survivalrate in aerated water varied from 85.5 to 100.0%. Seven polymorphic microsatellite loci were selectedfrom a set of 31 for paternity identification. The CPE (combined paternity exclusion probability) of twomicrosatellite loci was higher than 0.93 and the CPE for the seven loci used in this study was as high as0.9999. There were no unrelated individuals in five of the seven families analyzed and only one mixedindividual in F29 and F30. The overall admixture rate was very low (2.2%). In this study, we developedan optimized group mating system. Furthermore, we verified that for group mating systems there wereno mixed individuals in most families and the admixture rate of some families was very low accordingto the paternity analysis.

Keywords:Nile tilapiaFamilyMicrosatelliteParentage analysis

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Ketersediaan

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Informasi Detail

Judul Seri

Aquaculture Reports

No. Panggil

-

Penerbit

: Elsevier., 2016

Deskripsi Fisik

Aquaculture Reports 3 (2016) 45–50

Bahasa

English

ISBN/ISSN

-

Klasifikasi

NONE

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

3 ( 2016 )

Subjek

KIMIA

Info Detail Spesifik

-

Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

Lampiran Berkas

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