e-journal

Evidence of vertical transmission and tissue tropism ofStreptococcosis from naturally infected red tilapia (Oreochromis spp.)

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Streptococcosis is a highly problematic disease in the aquaculture of freshwater fishes, especially fortilapia. The possibility of vertical transmission of streptococcosis and the pattern of tissue tropism of thispathogen in various organs was examined in red tilapia (Oreochromis sp.). Healthy broodstock withoutany clinical signs of Streptococcus spp. were selected from a farm earlier reported to have the disease anda total of 10 pairs were forced spawned to provide samples of gametes and progeny for pathogen testing.A colorimetric LAMP assay was used to confirm whether the bacterial pathogens Streptococcus. agalactiaeand Streptococcus. iniae was present in samples of milt, unfertilized eggs, fertilized eggs, and offspring atvarious stages of development, as well as internal organs of broodstock (reproductive organs, gill, liver,spleen, kidney and brain) as well as samples of water from culture systems. The majority of samples ofmilt (9/10) and unfertilized eggs (7/10) collected from the broodstock were infected with S. iniae at thetime of spawning and was transmitted to all of their offspring. Nevertheless, when the same samplesof gametes were analyzed for S. agalactiae, they were all found to be negative but the pathogen wasfound to be present in some 10-day-old larval offspring (4/10). However, when the pathogenic presencewas analyzed from the reproductive organs of the parents, both S. agalactiae (11/20) and S. iniae (18/20)bacterium were common. Although, all broodstock were asymptomatic, almost all broodstock harbouredthe bacteria in many organs. Confirmation of vertical transmission of streptococcosis in tilapia meansthat intergenerational break cannot be used as a reliable and simple means of reducing or eliminatingthe prevalence of these difficult pathogens in aquaculture stock.

Keywords:TilapiaVertical transmissionSpecific pathogen freeStreptococcusTissue tropisma

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Informasi Detail

Judul Seri

Aquaculture Reports

No. Panggil

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Penerbit

: Elsevier., 2016

Deskripsi Fisik

Aquaculture Reports 3 (2016) 58–66

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

Lampiran Berkas

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