e-journal

Sample size matters in dietary gene expression studies—A case studyin the gilthead sea bream (Sparus aurata L.)

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One of the main concerns in gene expression studies is the calculation of statistical significance which inmost cases remains low due to limited sample size. Increasing biological replicates translates into moreeffective gains in power which, especially in nutritional experiments, is of great importance as individualvariation of growth performance parameters and feed conversion is high. The present study investigatesin the gilthead sea bream Sparus aurata, one of the most important Mediterranean aquaculture species.For 24 gilthead sea bream individuals (biological replicates) the effects of gradual substitution of fish mealby plant ingredients (0% (control), 25%, 50% and 75%) in the diets were studied by looking at expressionlevels of four immune-and stress-related genes in intestine, head kidney and liver. The present resultsshowed that only the lowest substitution percentage is tolerated and that liver is the most sensitivetissue to detect gene expression variations in relation to fish meal substituted diets. Additionally theusage of three independent biological replicates were evaluated by calculating the averages of all possibletriplets in order to assess the suitability of selected genes for stress indication as well as the impact ofthe experimental set up, thus in the present work the impact of FM substitution. Gene expression wasaltered depending of the selected biological triplicate. Only for two genes in liver (hsp70 and tgf) significantdifferential expression was assured independently of the triplicates used. These results underlined theimportance of choosing the adequate sample number especially when significant, but minor differencesin gene expression levels are observed.

Keywords:Sample sizeGene expressionFish meal replacementImmune responseGilthead sea breama

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Informasi Detail

Judul Seri

Aquaculture Reports

No. Panggil

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Penerbit

: Elsevier., 2016

Deskripsi Fisik

Aquaculture Reports 3 (2016) 82–87

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

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