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Mixture design analysis of solvent extractor effects on epicatechin, epigallocatechin gallate, epigallocatechin and antioxidant activities of the Camellia sinensis L. leaves

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a b s t r a c t:
This paper reports an RP-HPLC-UV-DAD study of the effects of four solvents and their mixtures on the extraction and antioxidant activities of three main catechins, epicatechin (EC), epigallocatechin gallate (EGCG) and epigallocatechin (EGC) of Camellia sinensis L. leaves for two harvests. The extraction efficiency solvent was measured by the chromatographic peak areas. The results showed that the relative abundance of the catechins in the second harvest is somewhat larger than in the first one, although there is no significant difference at the 95% level. The relative abundance found for EGCG is greater than for EGC which is greater than EC for all solvent mixtures. According to the mixture models, the maximum values of relative abundances of EGCG, EGC and EC can be obtained with a (70:30 v/v) ethanol:ethyl acetate binary mixture and the antioxidant activities with a (55:25:20 v/v/v) ethanol:ethyl acetate:dichloromethane ternary mixture.

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Ketersediaan

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Informasi Detail

Judul Seri

Analytical Chemistry

No. Panggil

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Penerbit

: Elsevier., 2014

Deskripsi Fisik

Analytical Chemistry Research 2 (2014), 23-29

Bahasa

English

ISBN/ISSN

Doi: 101016/j.ancr.2

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

(2014), 2

Subjek

ANALISIS KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

deliza

Versi lain/terkait

Lampiran Berkas

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