e-journal
Live prey enrichment and artificial microdiets for larviculture ofAtlantic red porgy Pagrus pagrus
In the first experiment the effects of rotifer enrichment and feeding frequency on larval performanceof red porgy Pagrus pagrus were studied. Larvae (2 days post-hatching = 2 dph) were fed s-type rotifers(∼20 rotifers/mL) enriched with one of the four different treatment media: Rotifer Diet (microalgae Nan-nochloropsis oculata and Tetraselmis chuii), DHA Protein Selco, Algamac 3000 (Schizochytrium sp.) andAlgamac + ARA (arachidonic acid). Larvae were fed daily at full ration or twice daily at half ration. Larvalgrowth and survival (mean = 22.8%) were satisfactory through 16 dph under all treatments; however,resistance to hyposaline challenge (Survival Activity Index = SAI) was positively correlated (P < 0.01) withDHA concentration of rotifers, and SAI appeared highest in the Algamac + ARA treatment. In the secondexperiment the effects of Artemia enrichment on larval performance were compared from 18 dph throughpre-metamorphosis (33 dph). Larvae were fed Artemia (0.5–3.0/mL) enriched with two different mediaAlgamac 3000 and DC DHA Selco, or unenriched Artemia (control). Both media improved DHA levels inArtemia and growth and survival (36.7–54.6%) of larvae, while larvae fed unenriched Artemia showedpoor growth and survival (5.2%). In the third experiment a University of North Carolina Wilmingtonmicrobound diet (MBD) and two commercial microdiets (Gemma Micro and Otohime) were evaluated.The MBD contained different protein sources (i.e., menhaden, squid and krill meal, soy protein concen-trate) and attractants. Beginning 16 dph, live feeds and microdiets were co-fed to three treatment groupsof larvae: (1) Gemma, (2) MBD, and (3) Otohime. Larval performance on the UNCW-MBD was comparableto the commercial microdiets, with no significant differences in larval survival, DHA, or total n-3 PUFAcontent through 32 dph. Results delineate more effective rearing protocols for larviculture of Atlantic redporgy juveniles.
Keywords:Red porgyLive prey enrichmentRotifersArtemiaMicrodietsMicrobound dietsEssential fatty acids
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