The cloning of a heat shock protein 90 gene and expression analysisin Botia reevesae after ammonia-N exposure and Aeromonashydrophila challenge

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The cloning of a heat shock protein 90 gene and expression analysisin Botia reevesae after ammonia-N exposure and Aeromonashydrophila challenge

Chuanjie Qin [et al.] - Nama Orang;

The objective of this study was to clone the full-length cDNA of Heat shock protein 90 (HSP90) in Botiareevesae and to determine the effects of pathogenic bacterial challenge after acute sublethal ammoniaexposure on HSP90 expression. The HSP90 cDNA of B. reevesae contained 2358 bp, including a 1947 bpopen reading frame, a 36 bp 5-untranslated region (UTR), a 375 bp 3-UTR. Sequence comparisons indi-cated that the predicted amino acid sequence of B. reevesae HSP90 showed a high degree of similaritywith other known HSP90 genes, and contained five HSP superfamily signatures, thus suggesting that B.reevesae HSP90 is a cytosolic member of the HSP90 family. Quantitative real-time PCR analysis revealedthat HSP90 transcripts could be detected in all of the tissues tested, and was strongly expressed in liver, gilland kidney tissues (p < 0.05) of B. reevesae after sublethal ammonia-N exposure and Aeromonas hydrophilachallenge. Additionally, following sublethal ammonia-N exposure and A. hydrophila challenge, expressionof HSP90 mRNA transcripts was increased in gill and kidney tissues by 6–24 h (p < 0.05), and was morereduced in the liver than the levels observed in response to ammonia-N exposure or A. hydrophila chal-lenge alone. This result indicated that after ammonia-N stress, B. reevesae could trigger elevated HSP90expression in specific tissues to respond to pathogenic bacteria.

Keywords:Heat shock protein 90cDNABotia reevesaeAmmonia-NStressa

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Judul Seri: Aquaculture Reports
No. Panggil: -
Penerbit: : Elsevier., 2016
Deskripsi Fisik: Aquaculture Reports 3 (2016) 159–165
Bahasa: English
ISBN/ISSN: -
Klasifikasi: -
Tipe Isi: -
Tipe Media: -
Tipe Pembawa: -
Edisi: -
Subjek: KIMIA
Info Detail Spesifik: -
Pernyataan Tanggungjawab: Wati/Agus

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FULL TEXT:The cloning of a heat shock protein 90 gene and expression analysisin Botia reevesae after ammonia-N exposure and Aeromonashydrophila challenge

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