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Rotifers enriched with a mixed algal diet promote survival, growthand development of barramundi larvae, Lates calcarifer (Bloch)
Fulfilling the nutritional requirements of marine fish larvae, including barramundi (Lates calcarifer),remains a bottleneck for optimal commercial hatchery production. This study investigated the effect ofmixed algal diets for the enrichment of rotifers on the survival, growth, development and stress resistanceof barramundi larvae. Four groups of 2–13 days-post-hatch (dph) barramundi larvae were fed rotifersenriched with either Nannochloropsis oculata, Chlorella vulgaris, or a blend of 50:50 or 75:25 of N. oculatato C. vulgaris (based on dry weight, DW), respectively. The highly unsaturated fatty acids (HUFAs) docosa-hexaenoic acid (DHA), eicosapentaenoic acid (EPA) and arachidonic acid (ARA) were used as qualitativeproxies of the feed. The barramundi larvae fed with the 50:50 diet outperformed all other treatments,with significantly greater larval length (P < 0.05), eye diameter (P < 0.05) and body depth (P < 0.05) at 10dph, a trend conserved over the whole growth period. The barramundi larvae fed the 50:50 diet alsoachieved the highest flexion percentage at 10 dph (P < 0.05) and scored the highest survival past thestress test (85%; P < 0.05). The best performing treatment had the second highest level of DHA whichsuggests that while DHA is an important fatty acid for barramundi larvae it is likely its interaction withother EFA and monounsaturated fatty acid also plays a critical role in their development. Overall, blendingmicroalgae species can enhance the balance of dietary fatty acid for barramundi larvae which in turn canlead to better growth, faster development and higher stress resistance when compared to monospecificmicroalgal diets.
Keywords:MicroalgaeLive feedBarramundiEssential fatty acidsEnrichmenta
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