e-journal

Isolation of probiotic bacteria from the hybrid South American catfishPseudoplatystoma reticulatum × Pseudoplatystoma corruscans(Siluriformes: Pimelodidae): A haematological approach

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This study investigated bacterial strains with probiotic potential isolated from the middle portionof healthy hybrid surubim catfish foregut (Pseudoplatystoma reticulatum female × P. corruscans male).Twenty surubims weighing 1.5 ± 0.3 kg were used for bacterial isolation. In total, 41 strains of bacteriawere selected in vitro. Ten strains had inhibition zones >10 mm against Aeromonas hydrophila. Five of thosestrains presented inhibition zones > 9 mm against other pathogenic bacteria and reached concentrationsgreater than 105CFU mL−1in tubes containing de Man, Rogosa and Sharpe (MRS) medium. In particular,Weissella cibaria (P36) reached 106CFU mL−1in MRS and was able to reduce the pH of the medium to3.85. In the in vivo intestinal colonization studies, 72 healthy hybrid surubims were fed with a commercialdiet supplemented with probiotic W. cibaria for 15 days. Changes in gut community composition werethen analyzed, and probiotic profile of W. cibaria was determined molecularly by amplification of rRNA16S gene was performed using PCR. Compared to control fish, W. cibaria-supplemented fish showed anincrease in RBC. These results show the efficacy of our haematological approach to probiotic screeningin hybrid sorubim.

Keywords:Fish nutritionSorubimLactic acid bacteriaWeissela cibariaa

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Informasi Detail

Judul Seri

Aquaculture Reports

No. Panggil

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Penerbit

: Graduate School of Economics, Waseda University., 2016

Deskripsi Fisik

Aquaculture Reports 3 (2016) 166–171

Bahasa

English

ISBN/ISSN

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Klasifikasi

NONE

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

3 ( 2016 )

Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

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