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Direct electron transfer biosensor for hydrogen peroxide carrying nanocomplex composed of horseradish peroxidase and Au-nanoparticle Characterization and application to bienzyme systems

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a b s t r a c t:
A reagentless electrochemical biosensor for hydrogen peroxide was fabricated. The sensor carries a monolayer of nanocomplex composed of horseradish peroxidase and Au-nanoparticle, and responds to hydrogen peroxide through the highly efficient direct electron transfer at a mild electrode potential
without any soluble mediator. Formation of the nanocomplex was studied with visible spectroscopy and size exclusion chromatography. The sensor performance was analyzed based on a hydrodynamic electrochemical technique and enzyme kinetics. The sensor was applied to fabrication of sensors for
glucose and uric acid through further modification of the nanocomplex-carrying electrode with the corresponding hydrogen peroxide-generating oxidases, glucose

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Ketersediaan

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Informasi Detail

Judul Seri

Analitycal Chemistry

No. Panggil

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Penerbit

: Elsevier., 2015

Deskripsi Fisik

Analitycal Chemistry Research 5 (2015), 1-8

Bahasa

English

ISBN/ISSN

Doi: 101016/j.ancr.2

Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

-

Edisi

(2015), 5

Subjek

ANALISIS KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

deliza

Versi lain/terkait

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