Cloud point extraction for analysis of antiretrovirals in human plasma by UFLC-ESI-MS/MS

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Cloud point extraction for analysis of antiretrovirals in human plasma by UFLC-ESI-MS/MS

Gabriel A. Hunzicker [et al.] - Nama Orang;

a b s t r a c t:
An analytical methodology based on cloud point extraction (CPE) coupled to Ultra-Fast Liquid Chromatography and electrospray tandem mass espectrometry (UFLC-MS/MS) was developed for analysis of Abacavir (ABC), Efavirenz (EFV), Lamivudine (3 TC) and Nelfinavir (NFV) in human plasma. It is the first time that CPE was used for extraction of antiretrovirals (ARV) from plasma. The effects of relevant physicchemical variables on analytical response of each ARV, including pH, surfactant concentration, equilibration time and temperature, were study and optimized; as well as its coupling to UFLC-ESI-MS/MS. Under optimized conditions, the resulting methodology was as follows: a 500 mL aliquot of human plasma was diluted with 2 mL deionized water in a 10 mL centrifuge tube. A 500 mL aliquot Triton X-114 5% w/v was added and homogenized using a vortex stirrer. The resulting cloudy solution was kept at 65 C for 20 min for promoting the condensation of surfactant micelles. Then it was centrifuged at 3000 g for 5 min for separation of the surfactant-rich phase. After discarding the aqueous supernatant, 400 mL ACN were added to the remaining surfactant rich phase and centrifuged in order to precipitate proteins and separate them. A 150 mL aliquot of the supernatant was transferred to 2 mL vial and further diluted with 400 mL deionized water. A 30 mL aliquot of the so-prepared solution was injected and analyzed into the UFLC-MS/MS. The method detection limits for ABC, EFV, 3 TC and NFV under optimized conditions were 31, 77, 57 and 21 ng mL1, respectively. The RSD% for the studied analytes were

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Informasi Detail

Judul Seri: Analitycal Chemistry
No. Panggil: -
Penerbit: : Elsevier., 2015
Deskripsi Fisik: Analitycal Chemistry Research 6 (2015), 1-8
Bahasa: English
ISBN/ISSN: Doi: 101016/j.ancr.2
Klasifikasi: -
Tipe Isi: -
Tipe Media: -
Tipe Pembawa: -
Edisi: (2015), 5
Subjek: ANALISIS KIMIA
Info Detail Spesifik: -
Pernyataan Tanggungjawab: deliza

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FULLTEXT: Cloud point extraction for analysis of antiretrovirals in human plasma by UFLC-ESI-MS/MS

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