e-journal

Characterization of Recombinant Vaccinia Viruses by MLPA Technology

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Abstract:
Recombinant vaccinia viruses are popular tools for the delivery of heterologous genes for vaccination purposes. Vaccinia viruses are generally classified as risk group 2 biological agents (in accordance to directive 2000/54/EC), with the attenuated strain Modified vaccinia virus Ankara (MVA) being classified into risk group 1. For the analytical surveillance of genetic engineering operations with vaccinia viruses, developing methods that enable the differentiation between risk group 2 vaccinia strains and MVA and that allow the identification of possibly inserted heterologous genes and their integration loci is important. This article describes a method based on multiplex ligation-dependent probe amplification (MLPA), which has been customized to simultaneously check 13 different vaccinia viral genome sequences. The presence or absence of MLPA
products points towards the utilized vaccinia viral backbone and the integration loci of heterologous genes.

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Ketersediaan

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Informasi Detail

Judul Seri

Applied Biosafety

No. Panggil

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Penerbit

: Applied Biosafety., 2014

Deskripsi Fisik

Applied Biosafety Vol. 19, No. 3, 2014, 132-140

Bahasa

English

ISBN/ISSN

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Klasifikasi

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Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

Vol. 19, No. 3, 2014

Subjek

Biosafety Terapan

Info Detail Spesifik

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Pernyataan Tanggungjawab

deliza

Versi lain/terkait

Lampiran Berkas

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