e-journal

Biochemical and Physiological Studies of Ischemia-Reperfusion in (Doca)-Salt Hypertensive Rat Heart

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Using a (DOCA)-salt arterial hypertension rat model we aimed to test on isolated, ischemised and reperfused rat heart,
physiological parameters as well as biochemical markers, both in myocardium and in plasma, in order to characterize
modifications induced by DOCA-salt treatment. In our study we used 20 rats of 10 months old divided into two groups of 10 rats each: control and treated. The treated group has received for 4 weeks 2 subcutaneous injections/week with DOCA (20 mg/b.w. in solution of 0.9% NaCl+0.2% KCl) according to the protocol, and 10-14 days since the last dose of treatment, the animals have been sacrificed. Coronary flow (CF), heart rate (HR) and left ventricle developed pressure (LVDP) have been measured on isolated and perfused Langendorff heart system at a constant pressure and total lipids, cholesterol, HDL and LDL, lipid peroxides as well as GGT, LDH, CK have been assessed using standard biochemical methods and Randox and Sigma kits. As results, there was an increase in C.F, H.R. and LVPD in DOCA-salt treated hearts, accounting for an intense mechanic cardiac activity generated by the hypertension following DOCA-salt treatment, accompanied by an increase in lipid peroxide, in CK and LDH as markers of myocardial lesion and a reduction of GGT activity in myocardial tissue probably due to hypoxia generated by the cardiac insufficiency following the increase in arterial blood pressure and a decrease in antioxidant potential. DOCA-salt hypertension is associated with rapid development of high blood pressure, vascular growth, vascular stiffening, and cardiac hypertrophy with increasing in mechanical activity of the heart, and a decrease in GGT activity and in antioxidant potential.

Keywords: deoxicorticosterone acetate, arterial hypertension, rat heart, ischemia-reperfusion, Gamma Glutamyl Transferase, oxidative stress

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Ketersediaan

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Informasi Detail

Judul Seri

Agriculture and Agricultural Science Procedia

No. Panggil

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Penerbit

: Elsevier., 2015

Deskripsi Fisik

Agriculture and Agricultural Science Procedia 6 ( 2015 ) 254 – 258

Bahasa

English

ISBN/ISSN

doi: 10.1016/j.aaspr

Klasifikasi

NONE

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

6 ( 2015 )

Subjek

Pertanian

Info Detail Spesifik

-

Pernyataan Tanggungjawab

Wati/Agus

Versi lain/terkait

Lampiran Berkas

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