e-journal
Automated Recovery of the Center of Rotation in Optical Projection Tomography in the Presence of Scattering
Abstract—Finding the center of rotation is an essential step for accurate 3-D reconstruction in optical projection tomography. Unfortunately,
current methods are not convenient since they require either prior scanning of a reference phantom, small structures of high intensity existing in the specimen, or active participation during the centering procedure. To solve these problems this paper proposes a fast and automatic center of rotation search method making use of parallel programming in graphics processing units. Our method is based on a two step search approach making use only of those sections of the image with high signal-to-noise ratio. We have tested this method both in nonscattering ex vivo samples
and in in vivo specimens with a considerable contribution of scattering such as Drosophila melanogaster pupae, recovering in all cases the center of rotation with a precision 1/4 pixel or less.
Index Terms—Center of rotation, optical projection tomography (OPT), parallel reconstruction, scattering.
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