e-book

Sample Preparation in LC‐MS Bioanalysis

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Sample preparation is a pivotal part of the integral LC‐MS bioanalysis, which has been heavily employed in the determination of drugs, drug metabolites, biomarkers, and other molecules of interest in various biological matrices (e.g. fluids or tissues) for decades. It has been playing an important role in a variety of human healthcare studies, ranging from drug discovery and development, therapeutic drug monitoring, to biomarker analysis. While highly sophisticated LC‐MS systems with better sensitivity and higher bioanalytical throughput have been continuously introduced, challenges that remain unchanged are the sample preparation prior to LC‐MS quantitation, for which data quality has direct impact on study conclusion.
The purpose of sample preparation is not only to make the analyte(s) of interest available in sample extracts at an appropriate concentration for MS detection but also to remove interfering matrix elements (e.g. phospholipids and salts) that, if not addressed properly, can alter MS response (e.g. signal suppression). In quantitative LC‐MS bioanalysis, clean sample extracts means: (i) better
chromatography, (ii) lower limit of quantification, (iii) decreased assay variability (due to reduced matrix effects), (iv) less chance of false‐positive/negative results, (v) longer column lifetime, (vi) less instrument downtime, and (vii) minimized costs in manpower and equipment maintenance, etc. In practice, the best sample preparation strategies should always be considered, evaluated, and implemented whenever possible in developing a robust quantitative LC‐MS bioanalytical method
As a companion for the previously published Handbook of LC‐MS Bioanalysis: Best Practice, Experimental Protocols and Regulations (Li, Zhang, and Tse, 2013, Wiley), the current book is to provide a timely and comprehensive update along with representative experimental protocols on all important sample preparation techniques for quantitative LC‐MS bioanalysis of small and large molecules. The 26 chapters of the book are divided into three parts. The first part of the book is focused on not only the basic but also the contemporary sample preparation techniques in LC‐MS bioanalysis. These include Protein Precipitation, Liquid–Liquid Extraction, and Solid‐Phase Extraction (Chapter 1), Online Extraction and Column Switching (Chapter 2), Equilibrium Dialysis, Ultracentrifugation, and Ultrafiltration (Chapter 3), Phospholipid Depletion (Chapter 4), Salting‐out Assisted Liquid–Liquid Extraction (SALLE) (Chapter 5), Supported Liquid Extraction (SLE) (Chapter 6), Immunocapture (Chapter 7), Microextraction (Chapter 8), Microsampling (Chapter 9), Extraction via Nanomaterials (Chapter 10), Extraction via Molecularly Imprinted Polymers (MIP) (Chapter 11),
Stir‐bar Sorptive Extraction (Chapter 12), Monolithic Spin Column Extraction (Chapter 13), Aptamer‐based Sample Preparation (Chapter 14), and Sample Extraction via Electromembranes (Chapter 15).
In Part II, the current sample preparation techniques for LC‐MS bioanalysis of biological sample matrices other than common whole blood, plasma, or serum are discussed in detail along with experimental protocols. These matrices include but are not limited to Tissues, Hair, Nail, Skins, and Bones (Chapter 16), Peripheral Blood Mononuclear Cells (Chapter 17), Urine, Cerebrospinal Fluid, Synovial Fluid, Sweat, Tears, and Aqueous Humor (Chapter 18), and Liposomal Samples (Chapter 19).
Part III of the book is focused on sample preparation for LC‐MS bioanalysis of challenging molecules. This part starts with some Key Pre‐analytical Considerations in Quantitative LC‐MS Bioanalysis (Chapter 20), which is followed by Derivatization strategies for enhancing assay sensitivities in quantitative LC‐MS bioanalysis of molecules with poor ionization efficiency (Chapter 21). Sample preparation for quantitative LC‐MS bioanalysis of Lipids is captured in Chapter 22. In Chapter 23, detailed instructions and associated stepwise protocols are provided for
LC‐MS bioanalysis of peptides. Expanding from peptides, detailed instructions of sample preparation for LC‐MS bioanalysis of Proteins, Oligonucleotides, and Antibody–drug Conjugates (ADCs) are captured in Chapters 24, 25, and 26, respectively

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Informasi Detail

Judul Seri

Wiley Series on Pharmaceutical Science and Biotechnology: Practices Applications and Methods

No. Panggil

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Penerbit

Hoboken : John Wiley & Sons, Ltd.., 2019

Deskripsi Fisik

387 hlm

Bahasa

English

ISBN/ISSN

9781119274308

Klasifikasi

NONE

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

-

Subjek

BIOTEKNOLOGI
Liquid Chromatography

Info Detail Spesifik

Bagi pemustaka yang membutuhkan fulltext bisa hubungi Perpustakaan Universitas Riau

Pernyataan Tanggungjawab

Wenkui Li, Wenying Jian, and Yunlin Fu

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