Sample preparation is a pivotal part of the integral LC‐MS bioanalysis, which has been heavily employed in the determination of drugs, drug metabolites, biomarkers, and other molecules of interest in various biological matrices (e.g. fluids or tissues) for decades. It has been playing an important role in a variety of human healthcare studies, ranging from drug discovery and development, th…
Accurate and specific analysis of target molecules in complex biological matrices remains a significant challenge, especially when ultra-trace detection limits are required. Liquid chromatography with mass spectrometry is often the method of choice for bioanalysis. Conventional sample preparation and clean-up methods prior to the analysis of biological fluids such as liquid–liquid extracti…