The maize mutant gene Vestigial glume 1 (Vg1) has been fine-mapped to a narrow region by ap-based cloning and the candidate gene for Vg1 spanned 19.5 kb. Here we report Vg1 genomic fosmid library construction and screening. The fosmid library of Vg1 consisted of 574,000 clones with an average insert size of 36.4 kb, representing 7.9-fold coverage of themaize genome. Fosmid stability assays indi…