A real-time PCR assay for detection and quantification of Botrytis cinerea in…

A reliable EvaGreen-based qPCR assay was developed for the relative quantification of fungal and plant DNAs in the Botrytis cinerea-Pelargonium× hortorum pathosystem. Primers, designed on RPB2 (DNA-dependent RNA polymerase subunit II) gene sequences directed the amplification of a specific 149 bp product for B. cinerea. No cross-reactivity was observed in DNA extracted from several nontarget f…