The cloning of a heat shock protein 90 gene and expression analysisin Botia …

The objective of this study was to clone the full-length cDNA of Heat shock protein 90 (HSP90) in Botiareevesae and to determine the effects of pathogenic bacterial challenge after acute sublethal ammoniaexposure on HSP90 expression. The HSP90 cDNA of B. reevesae contained 2358 bp, including a 1947 bpopen reading frame, a 36 bp 5-untranslated region (UTR), a 375 bp 3-UTR. Sequence compariso…