Washout Kinetics of Viral Vectors from Cultured Mammalian Cells

Abstract: The handling of infectious viral vectors such as adenovirus type 5 (Ad5), lentivirus (HIV1), and vaccinia virus (VV) requires biosafety level 2 (BSL-2) containment. This also applieso modified vaccinia Ankara (MVA) and adeno-associated virus type 2 (AAV2) provided that the insert involves additional hazard. To transfer infected cells to the lower BSL-1 containment,viral particles hav…