During the usage of Rhizopus oryzae whole cell instead of immobilized enzyme for biodiesel production, the intracellular lipase was found to have 1,3-positional specificity when used to catalyze methanolysis of triglycerides. Thus, the application of R. oryzae whole cell in biodiesel production from triglycerides is restrained to some extent. However, it might be a promising catalyst for biod…