In the presence of Britton-Robinson buffer solution (pH ¼ 9.5) and surfactant of Tween-80,fluorescence intensity of calcein was quenched by Pd2þ. However, the fluorescence intensity can be enhanced after adding a certain concentration of L-arginine, and the rate of the enhancement showed a good liner relationship with the added amount of L-arginine. We then established a fluorescence spectro…