Flavobacterium psychrophilum is the causative agent of bacterial cold-water disease (BCWD) and causes severe loss of aquaculture production of ayu sweetfish Plecoglossus altivelis altivelis (Temminck & Schlegel, 1846) in Japan (Wakabayashi, Toyama & Iida 1994). The characteristics of BCWD in ayu are different from those of BCWD in salmonid fishes. BCWD in ayu generally occurs when water tempera…