Development and validation of a high performance liquid chromatography method…

a b s t r a c t The therapeutic benefit of phosphorothioate oligod eoxynucleotides (PS-ODN) containing immune stimulatory sequences has been demonstrated in animal models of cancer and infection. Several tools are available for the determination of these oligonucleotides in biological samples and pharmaceutical preparations, including UV spectroscopy, dye binding, isotopic tracing, capillary g…