Pollen and seed transmission of Cucumber green mottle mosaic virus in cucumber

Cucumber green mottle mosaic virus (CGMMV) is transmitted mechanically in cucurbits, but whether it is transmitted via pollen to healthy plants and onto the subsequent generation of seedlings is unknown. Greenhouse experiments were conducted to investigate the importance of this route of infection. Cucumber seedlings at the 3-true-leaf stage were mechanically inoculated with CGMMV. At anthesis,…