While the yield potential of rice has increased but little is known about the impact of breeding on grain quality, especially under different levels of N availability. In order to investigate the integrated effects of breeding andNlevels on rice quality 12 japonica rice cultivars bred in the past 60 years in the Yangtze River Basinwere usedwith three levels of N: 0 kg N ha−1, 240 kg N ha−1,…