b-glucan binding protein (bGBP), a pattern recognition protein was purified from the haemolymph of freshwater prawn Macrobrachium rosenbergii by heparin affinity chromatography that showed a single band in native gradient PAGE. The b-glucan binding property of the purified protein was confirmed in a phenoloxidase (PO) assay, where addition of bGBP along with b-glucan increased the specific P…