This study aims at immunochemical characterization of plasma vitellogenin (VTG), development of anheterlogous VTG ELISA and to relate seasonal variation in plasma VTG and estradiol-17 (E2) levels withovarian growth (gonadosomatic index, GSI) in Olyra longicaudata (McClelland, 1842), a rare hill-streamcatfish endemic to North East India. On native PAGE, plasma from E2-injected male, vitellogeni…