Modeling development of inhibition zones in an agar diffusion bioassay

A two-temperature agar diffusion bioassay is commonly used to quantify theconcentration of nisin using Micrococcus luteus as the indicator microorganism. A finite element computational model based on Fick’s second law of diffusionwas used to predict the radius of the inhibition zone in this diffusion bioassay.The model developed was used to calculate nisin concentration profiles as a function…