Expanded metabolite coverage of Saccharomyces cerevisiae extract through impr…

We present an improved extraction and derivatization protocol for GCeMS analysis of amino/non-amino acids in Saccharomyces cerevisiae. Yeast cells were extracted with chloroform: aqueous-methanol (1:1, v/ v) and the resulting non-polar and polar extracts combined and dried for derivatization. Polar and nonpolar metabolites were derivatized using tert-butyldimethylsilyl (t-BDMS) dissolved in …