This research aims to get the optimum combination of Hydraulic Retention Time and addition of inoculums in tofu’s wastewater treatment using anaerobic system. Research was done using Central Composite Design (CCD) with two factors, which were Hydraulic Retention Time (12; 18; and 24 hours) and addition of inoculums (5%; 10%; and 15%). The desired responses was percentage decrease of COD, BOD,…