AP4 method for two-tube nested PCR detection of AHPND isolates ofVibrio parah…

Our previous work on the mechanism of virulence for the unique isolates of Vibrio parahaemolyticus thatcause acute hepatopancreatic necrosis disease (VPAHPND)revealed that it was mediated by a binary Pir-liketoxin pair ToxA and ToxB. These toxins are located on the pVA plasmid, a plasmid carried by AHPND-causing strain of V. parahaemolyticus with a size of approximately 69 kbp. Using the coding…