The present paper describes the development of a high performance liquid chromatographyultraviolet (HPLC-UV) detection method for quantitative determination of peimine and peiminine in Fritillariae Thunbergii Bulbus (FTB). Separation was achieved using a conventional XBridge™Shield RP 18 column (250mm4.6mm,internal diameter 3.5 mm) with photodiode array detection at 190e400 nm for UV spectr…