e-journal

Exploration of Single Molecule Events in a Haloperoxidase and Its Biomimic: Localization of Halogenation Activity

---

The spatiotemporal resolution and the ever increasing sensitivity of single-molecule fluorescence spectroscopy (SMFS) give access to insights at the molecular level for a wide range of systems, while
such details were previously hidden in ensemble-averaged measurements. In particular, SMFS has provided kinetic and molecular understanding in bio- and chemocatalytic processes thanks to the possibility of performing ultrasensitive real-time dynamic studies under in situ conditions. Recently, several strategies have been developed for monitoring catalytic activity using fluorescence microscopy. One of these uses an active site that switches between different fluorescent states during its catalytic cycle. An alternative
scheme relies on fluorogenic substrates, thereby bypassing the issue of photobleaching. While a large assortment of fluorogenic probes is available for bulk activity measurements, only a few of them are suitable for SMFS. Exploration of single molecule events with such probes in catalytic systems has thus so far been limited. This work introduces a simple assay to monitor individual oxidation events at varying distances from a haloperoxidase biocatalyst and its inorganic biomimic.

---

Ketersediaan

Tidak ada salinan data

Informasi Detail

Judul Seri

J. AM. CHEM. SOC.

No. Panggil

-

Penerbit

: American Chemical Society., 2008

Deskripsi Fisik

J. AM. CHEM. SOC. 2008, 130, 13192–13193

Bahasa

English

ISBN/ISSN

-

Klasifikasi

-

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

-

Subjek

KIMIA

Info Detail Spesifik

-

Pernyataan Tanggungjawab

agus

Versi lain/terkait

Lampiran Berkas

Komentar

---

Anda harus masuk sebelum memberikan komentar