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The Impact of Food Bio-Actives on Gut Health
This book is a product of the InfoGest COST Action FA 1005. This Action was granted by COST in the domain of Food and Agriculture and coordinated by the French National Institute for Agricultural Research (INRA). Every day our gastrointestinal tract (GIT) is exposed to many different food
components. These food components can be processed, digested and eventually transported across the intestinal tract and can have a variety of direct and indirect effects, both positive and negative, on our health. In vivo assessments of the impacts of food components (or food bioactives) on health are not trivial and are not practical when examining more than just a few components. Thus, in many instances it is necessary to employ in vitro and ex vivo models as part of a funnelled approach to
identify the components that merit particular attention.
In this book, we describe the in vitro and ex vivo models which can be used to investigate benefi cial or detrimental effects of digested food products. The models that we describe in this book include those used to study digestion and fermentation in the small and large intestine (Parts I and VI), models used to investigate absorption (e.g. Ussing chamber, epithelial cell systems) in Part II and Part V and the immune and enteroendocrine responses (e.g. macrophages, dendritic cells, cocultures)
in Parts III and IV . For each model, we provide you with background information, a general protocol with tips and tricks concerning their proper use, readouts provided by the systems, the applicability of the model with respect to food research and pros and cons of the model. Indeed, this book has been prepared for the particular benefi t of students/researchers who are not experienced in the use
of these models but are considering their use. It is hoped that the details provided, and the citations included, will allow you to identify the model(s) that best suit(s) your needs
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