Introgression of the high grain protein gene Gpc-B1 in an elite wheatvariety of Indo-Gangetic Plains through marker assisted backcrossbreeding

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Introgression of the high grain protein gene Gpc-B1 in an elite wheatvariety of Indo-Gangetic Plains through marker assisted backcrossbreeding

backcrossbreedingManish K. Vishwakarma [et al.] - Nama Orang;

Grain protein content (GPC) in wheat has been a major trait of interest for breeders since it has enormousend use potential. In the present study, marker-assisted backcrossing (MABC) was successfully used toimprove GPC in wheat cultivar HUW468. The genotype Glu269 was used as the donor parent for intro-gression of the gene Gpc-B1 that confers high GPC. In a segregating population, SSR marker Xucw108, withits locus linked to Gpc-B1 was used for foreground selection to select plants carrying Gpc-B1. Backgroundselection, involving 86 polymorphic SSR markers dispersed throughout the genome, was exercised torecover the genome of HUW468. For eliminating linkage drag, markers spanning a 10 cM region aroundthe gene Gpc-B1 were employed to select lines with a donor segment of the minimum size carryingthe gene of interest. Improved lines had significantly higher GPC and displayed 88.4–92.3 per cent ofthe recurrent parent genome (RPG). For grain yield, selected lines were at par with the recurrent par-ent HUW468, suggesting that there was no yield penalty. The whole exercise of transfer of Gpc-B1 andreconstitution of the genome of HUW468 was completed within a period of two and half years (five cropcycles) demonstrating practical utility of MABC for developing high GPC lines in the background of anyelite and popular wheat cultivar with relatively higher speed and precision.

Keywords:Grain protein contentTriticum aestivumMarker assisted selectionForeground selectionBackground selection

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Judul Seri: Current Plant Biology
No. Panggil: -
Penerbit: : Elsevier., 2014
Deskripsi Fisik: Current Plant Biology 1 (2014) 60–67
Bahasa: English
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Subjek: KIMIA
Info Detail Spesifik: -
Pernyataan Tanggungjawab: Wati/Agus

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FULL TEXT:Introgression of the high grain protein gene Gpc-B1 in an elite wheatvariety of Indo-Gangetic Plains through marker assisted backcrossbreeding

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