In the first experiment the effects of rotifer enrichment and feeding frequency on larval performanceof red porgy Pagrus pagrus were studied. Larvae (2 days post-hatching = 2 dph) were fed s-type rotifers(∼20 rotifers/mL) enriched with one of the four different treatment media: Rotifer Diet (microalgae Nan-nochloropsis oculata and Tetraselmis chuii), DHA Protein Selco, Algamac 3000 (Schizochy…
a b s t r a c t Heparin is a linear sulfated polysaccharide widely used therapeutically as an anticoagulant. It is also the starting material for manufacturing low-molecular-weight heparins (LMWH). Quality control of heparin and LMWH is critical to ensure the safety and therapeutic activity of the final product. However due to their complex and heterogeneous structure, orthogonal analytical te…
a b s t r a c t: The distribution of 19 azole class pesticides in hexane/aqueous–organic mixtures systems and rapeseed oil (or oil solution in hexane)/organic solvents has been studied at 20 ± 1 C. The distribution constants (P) and coefficients (D) between hydrocarbon and polar phase are calculated. It is found that all the studied pesticides are hydrophobic, i.e., in hexane–water syste…
a b s t r a c t: A comprehensive review with 185 references for the analysis of commonly prescribed members of an important class of drugs, non-sedating antihistamines (NSAs), is presented. The review covers most of the methods described for the analysis of cetirizine (CTZ), ebastine (EBS), fexofenadine (FXD), ketotifen (KET) and loratadine (LOR) in pure forms, in different pharmaceutical dosa…
a b s t r a c t Polychlorinated biphenyls (PCBs) are persistent organic pollutants in insulating oil of a large number of transformers. A rapid and economical analytical method to detect PCB contamination is still required. To address this issue, we propose here the first microfluidic screening method for PCB contamination in insulating oil. The insulating oil was pretreated using a multilayer…
a b s t r a c t Nitrated indolinyl photoprotecting groups are crucial tools extensively used in the study of neuronal signal transduction. Mononitrated photolabile protecting groups have been used effectively, however, recent advances in the introduction of a second nitro group have shown improvement in the photo efficiency of neurotransmitter (agonist) release, albeit, to varying extents, dep…
a b s t r a c t: A new ketamine coated silver electrode (KCSE) based on ketamine hydrochloride with sodium tetraphenylborate (KT-TPB) as electroactive material has been described. The influence of membrane composition,type of solvent mediators, kind of electroactive materials and interfering ions on the sensor was investigated.The sensor displays Nernstian response of 55.8 ± 0.3 mV/decade ove…
a b s t r a c t: A simple, ultra sensitive and selective method for the spectrophotometric determination of Ir3+ in various synthetic and environmental samples was reported. The analyte ions were collected on a membrane filter in the form of their red complex with 5-(2-benzothiazolyl-azo)8-hydroxy-quinoline (BTAHQ), and the absorption spectra of the colored membrane filters were acquired. Effe…
a b s t r a c t: In this paper, we describe a simple method for sampling, pre-concentrating, and separating volatile and semi-volatile components from two different cave atmospheres. Sampling is performed by capturing a volume of cave atmosphere in a Tedlar bag or Suma canister for sample storage and transport back to the laboratory. Loading a portion of the sample on a multi-bed sorption tr…
a b s t r a c t: This paper reports an RP-HPLC-UV-DAD study of the effects of four solvents and their mixtures on the extraction and antioxidant activities of three main catechins, epicatechin (EC), epigallocatechin gallate (EGCG) and epigallocatechin (EGC) of Camellia sinensis L. leaves for two harvests. The extraction efficiency solvent was measured by the chromatographic peak areas. The re…
a b s t r a c t Benign prostatic hyperplasia (BPH) is one of the most common chronic diseases affecting men and itincreases in both incidence and prevalence with age. This work presents a simple, sensitive and fast generic high performance thin layer chromatographic (HPTLC) method for the simultaneous determination of five drugs prescribed for the treatment of BPH. These drugs include the a1-a…
In order to explore the effect of water temperature on sex ratio and growth rate, we reared the juvenilesof Pelteobagrus fulvidraco, Pelteobagrus vachelli and the hybrids from P. fulvidraco (♀) × P. vachelli (♂) at6 different water temperature conditions (20, 23, 26, 29, 32 and 34◦C) in this study. The results showedthe population of male and female fish at the ratio of 1:1 when the fish…
a b s t r a c t: PBDEs are widely used brominated flame retardant, which are increasingly reported in the environment. MeO-PBDEs are structural analogs to PBDEs, and reported as natural products and novel pollutants present in the environment. Concentrations of thirteen PBDEs and eight MeO-PBDEs in a large number of commercial sales of hen eggs representing 15 different regions and household p…
One of the main concerns in gene expression studies is the calculation of statistical significance which inmost cases remains low due to limited sample size. Increasing biological replicates translates into moreeffective gains in power which, especially in nutritional experiments, is of great importance as individualvariation of growth performance parameters and feed conversion is high. The pre…
Aquaculture of the Eurasian perch, Perca fluviatilis, in recirculating systems has emerged over the pastdecades to become a significant way of diversification for inland areas in Europe. The development of sucha production relies partly on the improvement of growth performance (i.e., reducing production costs),which requires suitable genetic management of broodstocks and the development of sele…
Abstract This study examines possibilities in improving Guam’s economic independence. Growing economically and culturally sustainable industries benefit future generations. In this work bamboo,historically utilized on the island by the native CHamoru, is evaluated for industry and market potential specifically for Guam. Young adults of the island engaged to find creative possibilities for im…
Streptococcosis is a highly problematic disease in the aquaculture of freshwater fishes, especially fortilapia. The possibility of vertical transmission of streptococcosis and the pattern of tissue tropism of thispathogen in various organs was examined in red tilapia (Oreochromis sp.). Healthy broodstock withoutany clinical signs of Streptococcus spp. were selected from a farm earlier reported …
Abstract In the Adriatic, the importance in tourism of the small rowing and sailing boats, like the gajeta and other heritage vessels, is clearly relevant, as icons of heritage boats adorn brochures, logos, and their names are bequested to hotels and restaurants. As a symbol, the gajeta stands for the heritage of the island, and the ethics of the place; these constitute an intangible treasure …
Abstract SDBR is largely representative of the aforementioned archipelago, and its topography alone allows for species diversity. The demarcated divisions of SDBR have the following environmental traits. Eleven inhabited islands, including Heuksando and Hongdo, and eighty-nine uninhabited islands make up a total of one hundred islands and beaches. The coastline stretches 74.39 km long, and the…
Abstract This study assessed the spatial distribution of population growth of Marinduque, an island province in the heart of the Philippines. Based on the results, population of the province varies across time and space while densely populated areas are concentrated in coastal, flat and relatively flat regions. From 1990–2010, majority of the villages in the province experienced an increase i…
In this study, we assessed the suitable level of replacement of fishmeal with a blue green microalga,Arthrospira platensis in feed for the post larvae (PL) of the freshwater prawn, Macrobrachium rosenbergiiby evaluating the growth performance, prawn proximate composition, feed utilization parameters andthe activity of digestive enzymes. The prawns were fed 5 different diets: a control diet and …
Abstract : This paper explores a socio-economic impact of ferry provision in Zadar island archipelago in Croatia. Poor frequency, accessibility, and unaffordable ferry prices to smaller islands have had a detrimental impact on island communities’ sustainability. This prompts for asking what constitutes the socio-economic impact of ferry provision on remote and rural islands’ sustainability…
Abstract Sustainable management processes have undergone a shift from a top-down approach to a bottom-up approach. This bottom-up approach allows for a more apprehensive inclusion of stakeholders. In traditional hierarchical societies a combination of both is considered more desirable. This combination is described as a participatory approach that allows for bi-directional knowledge sharing. Th…
Using vitellogenin (Vtg) gene as a biomarker, the possible role of selected endocrine modulators(EMs) such as methyl farnesoate (MF), 17-hydroxy progesterone (17-OHP), biochanin-A, methio-nine enkephaline (ME) and arachidonic acid on female reproduction in crab Oziothelphusa senex senex(O. senex senex) was elucidated in this study. The injections were given on 1st and 7th day of experi-ment (…
The tambaqui (Colossoma macropomum) is a native fish species that is farmed most frequently and in thelargest quantities throughout Brazil. The high production of this species from fish farms has contributedto the occurrence of emerging parasites, which may compromise fish health and productivity. In a batchof 2500 tambaqui fry acquired for experimental farming procedures in Brazil, a mortality…
Abstract: Local economies and livelihoods, cultures, and sustainability around the world are being challenged by wide ranging social and environmental changes. Despite many negative impacts, these changes also bring opportunities to initiate and implement innovations. Island communities are experiencing the forefront of much such action, particularly since they are often highly local and loca…
Everyone is becoming more environmentally conscious and therefore, chemical processes are being developed with their environmental burden in mind. Of course, this also means that more traditional chemical methods are being replaced with new innovations. This includes new solvents. Solvents are everywhere, but should they be? They are used in most areas including synthetic chemistry, anal…
Recent progress in molecular biology leads to a better understanding of nature and natural life processes at the global and local scopes and opens new horizons for chemical and biological technology. Alkaloids, the subject of this book, are a group of very interesting and complex chemical and biological molecules having special tasks in the life machinery of our planet. They are also a good…
This book was written with the intent of providing a text suitable for use in both graduate and undergraduate courses on propulsion. The format is such that some overlap will occur when the book is thus used, but the author has found that the diversified background typically found in most graduate classes is such that some repetition of undergraduate material is appropriate. At the Universit…
Nurses have always had to have knowledge of drugs as they have been involved with their administration both in the hospital and the community setting, and they have also been involved in the education of patients in regard to drug therapy. However, with the advent of nurse prescribing, albeit from a limited list of drugs, and the increasing use of drug protocols, the need for nurses to hav…
Advancing Nursing Practice in Pain Management provides an important resource for all nurses seeking to develop their practice and move towards the pinnacle of the clinical career pathway in nursing. This book provides a wealth of insights and practice expertise with regard to caring for people in pain that will be of value to the individual generalist and specialist practitioner, the nurse …
Freshwater snail, Pila globosa is an edible mollusc which bears commercial and ethnomedicinal signifi-cance. During environmental aridity, P. globosa aestivates deep into the soil for four to five months. Wereport comparative analyses of immune associated parameters in the hemocytes and hemolymph of P.globosa during activity, experimental aestivation, arousal and starvation. Hemocyte is a major…
Freshwater snail, Pila globosa is an edible mollusc which bears commercial and ethnomedicinal signifi-cance. During environmental aridity, P. globosa aestivates deep into the soil for four to five months. Wereport comparative analyses of immune associated parameters in the hemocytes and hemolymph of P.globosa during activity, experimental aestivation, arousal and starvation. Hemocyte is a major…
The demand for animal protein, especially fish, is growing fast, perhaps due to the rapid expansion ofpopulations, increases in income and changes in eating habits and life styles. Capture fisheries, whichsupply over 70% of the fish for human consumption in Malaysia, are over-exploited or depleted. Theiryields have become stagnant over the last few decades and in some cases have even declined. …
Motile Aeromonas septicemia (MAS), caused by virulent clonal isolates of Aeromonas hydrophila (vAh), isemerging as a major disease in catfish (Ictalurus punctatus) aquaculture in the Southeastern United States.Predisposing conditions leading to vAh infection in catfish were however largely unknown. The objectiveof this study was to investigate factors that predispose catfish to vAh infection an…
Red sea bream is one of the most important aquaculture fish species in Japan. To improve the productivityof this fish during seed production, improved growth traits and reduced size variation are needed. In thisstudy, we assessed parental contribution of fast- and slow-growing individuals observed in two differentrearing phases in a mass production lot: (1) 50 dph reared in a tank and (2) 200 d…
Red sea bream is one of the most important aquaculture fish species in Japan. To improve the productivityof this fish during seed production, improved growth traits and reduced size variation are needed. In thisstudy, we assessed parental contribution of fast- and slow-growing individuals observed in two differentrearing phases in a mass production lot: (1) 50 dph reared in a tank and (2) 200 d…
A study was undertaken to evaluate some factors that are important for better management of the whitespot syndrome virus (WSSV). We examined the likelihood of that the mud crab (Scylla serrata) is a poten-tial carrier of WSSV in shrimp farms and then compared susceptibility of three shrimp species to WSSV,and finally tested the effects of shrimp stocking density and transmission routes on the i…
Lates calcarifer (barramundi or Asian seabass) has been farmed since the 1970s, yet despite its widespreadculture little has been documented on the species’ embryonic development and particularly how devel-opment relates to temperature. This is particularly the case for the Australian L. calcarifer genetic strain.Accordingly, embryonic development of fertilised barramundi eggs incubated at 26…
Environmental pH is one of the factors contributing to abiotic stress which in turn influences the growthand development of macroalgae. This study was conducted in order to assess the growth and physio-logical changes in Kappaphycus alvarezii under different pH conditions: pHs 6, ∼8.4 (control) and 9. K.alvarezii explants exhibited a difference in the daily growth rate (DGR) among the differe…
Crustaceans have a powerful non-specific immune mechanism that responds to pathogen invasion andtogether with cellular responses, generates powerful humoral factors such as antimicrobial peptides.Crustins are a diverse class of antimicrobial peptides that are expressed by the circulating haemocytesof crustaceans. Several isoforms of this molecule are reported and in this study, one isoform from…
Stress is one of the most critical factors in fish health. The response to stress in fish is characterized bythe stimulation of the hypothalamus, which results in the activation of the neuroendocrine system anda subsequent cascade of metabolic and physiological changes. The present paper deals with the stressresponses of water pH on certain biochemical indices and hematological parameters of ze…
Genes coding for interferon (IFN-) has been cloned and studied for functional analysis in many species.Labeo rohita commonly known as rohu, is a commercially important and widely cultured fish in South-East Asia. IFN- of rohu was amplified with primers based on conserved regions of other related species.5and 3end were amplified using RACE PCR. The PCR products were cloned and sequenced. T…
De-oiled groundnut oil cake (GOC) was bio-processed through solid state fermentation (SSF) for 15 days at34 ± 1◦C by a tannase producing yeast, Pichia kudriavzevii (GU939629) isolated from the gut of a freshwa-ter carp, Cirrhinus cirrhosus. SSF of GOC was effective in significantly (P < 0.05) reducing crude fibre contentand antinutritional factors (e.g., tannin, phytic acid and trypsin inhib…
Mud crabs from the genus Scylla have high commercial value and are considered as one of the highlysought luxury seafood items. Thorough understanding about their biology and mating behaviour is vitalin providing important information for a sustainable exploitation and future incorporation into the aqua-culture industry. The mating process of S. olivacea lasted 82.0 ± 10.8 h was divided into fo…
This study registers for the first time the copepod Acusicola sp. infesting the gills of farmed yellowtail tetraAstyanax altiparanae, as well as new information on gross pathology and gill alterations. Five specimens ofyellowtail tetra showing slow swimming, respiratory difficulty, scaleness and darkened skin were exam-ined. Fish were analysed in situ for ectoparasites diagnosis. Fragments of t…