We present an improved extraction and derivatization protocol for GCeMS analysis of amino/non-amino acids in Saccharomyces cerevisiae. Yeast cells were extracted with chloroform: aqueous-methanol (1:1, v/ v) and the resulting non-polar and polar extracts combined and dried for derivatization. Polar and nonpolar metabolites were derivatized using tert-butyldimethylsilyl (t-BDMS) dissolved in …
An electro-deposition approach was established to incorporate the gold nano-flakes onto the glassy carbon electrode in electrochemical cells (nano-Au/GC/ECCs). Using pulsed amperometric detection (PAD) without any gold oxidation for cleaning (non-oxidative PAD), the nano-Au/GC/ECCs were able to maintain their activity for oxidizing of carbohydrates in a normal alkaline medium. The reproducib…
This study demonstrates the influence of the solvent when thermospray flame furnace atomic absorption spectrometry (TS-FF-AAS) is employed for the determination of elements of low volatility, taking magnesium (Mg) as leading case. Several organic solvents/water solutions of different characteristics (density, surface tension, viscosity, etc.) and proportions were employed for the TS-FF-AAS a…
Pinot Noir red wines made by malolactic fermentation were studied for studying differences in their chemical profiles with help of a wide spectrum of grape-based and other chemical compounds used in winemaking. Determinations were made with capillary electrophoresis, liquid chromatography, and spectrometry to investigate carbohydrates, organic acids, aldehydes, anthocyanins, phenolic compoun…
Chemometric techniques such as partial least squares combined with discriminant analysis (PLS–DA) and artificial neural networks (ANN) analysis were used to enhance the detection, discrimination and quantification of chemical warfare agents simulants. Triethyl phosphate (TEP) mixed with commercial products in their original containers was analyzed through the container walls using fiber-op…
Analytical chemists and others in many disciplines frequently ask questions such as: What is this substance?; How concentrated is this solution?; What is the structure of this molecule? The answers to these and many other similar questions are provided by the techniques and methods of analytical chemistry. They are common to a wide range of activities, and the demand for analytical data of a…
The eighth edition of Fundamentals of Analytical Chemistry, like it predecessors. is an introductory textbook designed primarily for one - or two-semester course for chemistry majors. Since the publication of the seventh edition, the scope of analytical chemistry has continued to evolve, and in this edition we have included many applicalions to biology, medicine, materials science, ecology, fo…
Analytical chemistry is concerned with the chemical characterization of matter, both qualitative and quantitative. It is important in nearly every aspect of our lives because chemicals make up everything we use. This text deals with the principles and techniques of quantitative analysis, that is, how to determine how much of a specific substance is contained in a sample. You will learn how t…
Analytical chemistry is concerned with the chemical characterization of matter, both qualitative and quantitative. It is important in nearly every aspect of our lives because chemicals make up everything we use. This text deals with the principles and techniques of quantitative analysis, that is, how to determine how much of a specific substance is contained in a sample. You will learn how t…