Lehninger Principles of Biochemistry

Edisi

8th Edition

ISBN/ISSN

978-1-319-32234-2

Deskripsi Fisik

4381 hlm

Judul Seri

-

No. Panggil

-

Biochemistry

Edisi

Eighth Edition

ISBN/ISSN

978-1-4641-2610-9

Deskripsi Fisik

1227 hlm

Judul Seri

-

No. Panggil

-

Medical Biochemistry

A textbook is a snapshot of a field as it exists at the time of writing. Such ‘photographic’ metaphor is appropriate here, because biochemistry undergoes constant change; in the period since the publication of the 3rd edition it has probably changed faster than ever before. While core metabolic pathways remain largely unchanged, our understanding of underlying regulatory mechanisms is be…

Bioinorganic chemistry

This book covers material that could be included in a one-quarter or onesemester course in bioinorganic chemistry for graduate students and advanced undergraduate students in chemistry or biochemistry. We believe that such a course should provide students with the background required to follow the research literature in the field. The topics were chosen to represent those areas of bioinorg…

Biochemistry Free and Easy

Edisi

3rd ed.

ISBN/ISSN

-

Deskripsi Fisik

ix, 448 Hlm.

Judul Seri

-

No. Panggil

-

Biochemistry

Edisi

5th ed.

ISBN/ISSN

978-1-60831-412-6

Deskripsi Fisik

QP514.2.C48 - 612'.015--dc22

Judul Seri

Lippincott’s Illustrated Reviews

No. Panggil

-

Biochemistry

It was perhaps a mark of how successful the second edition of Instant Notes in Biochemistry was that we recall seeing a final year student avidly reading it even as he waited to have his viva with the External Examiner. Although we would strongly recommend to any student not to leave revision to such a very late stage, this experience alone proved the value of a concise book that focused on …

Biochemistry

Biochemistry is a field of enormous fascination and utility, arising, no doubt, from our own self-interest. Human welfare, particularly its medical and nutritional aspects, has been vastly improved by our rapidly growing understanding of biochemistry. Indeed, scarcely a day passes without the report of a biomedical discovery that benefits a significant portion of humanity. Further advances in t…

Accurate Quantitation of Ki67-positive Proliferating Hepatocytes in Rabbit Li…

Determination of hepatocyte proliferation activity is hampered by the presence of Ki67-positive non-parenchymal cells. We validated a multicolor immunohistochemical (IHC) approach using multispectral tissue and cell segmentation software. Portal vein branches to the cranial liver lobes of 10 rabbits were embolized, leading to atrophy of the cranial lobes and hyperplasia of the caudal lobes. …

H3K9 Trimethylation Precedes DNA Methylation during Sheep Oogenesis: HDAC1, S…

The oocyte, to become a fully mature gamete, has to acquire a correct pattern of DNA methylation on its genome; this epigenetic event represents the major point of the molecular mechanisms that occur during postnatal oogenesis. It is known that an intimate link exists between DNA methylation and histone posttranslational modifications, such as trimethylation of lysine 9 on histone 3 (H3K9me3),…

Upregulation of Vascular Endothelial Growth Factor Receptor-3 in the Spinal C…

We investigated the spatiotemporal expression of vascular endothelial growth factor receptor–3 (VEGFR-3) in the spinal cord of Lewis rats with experimental autoimmune encephalomyelitis (EAE), an animal model for multiple sclerosis. VEGFR-3 mRNA and protein were constitutively expressed in gray matter neurons and in a few white matter astrocytes. Induction of VEGFR-3 occurred predominantly …

Dynamic Expression Pattern of Neuro-oncological Ventral Antigen 1 (Nova1) in …

The present study aimed to evaluate the expression of neuro-oncological ventral antigen 1 (Nova1) in cerebral ischemia/ reperfusion (I/R) insults by immunohistochemistry. The focal cerebral I/R model was induced by right middle cerebral artery occlusion (MCAO) for 120 min followed by 1 day, 7 days, and 14 days of reperfusion in Sprague-Dawley (SD) rats. The results showed that Nova1 was expr…

Short-term Hypoxia Transiently Increases Dopamine β-Hydroxylase Immunoreacti…

Under long-term hypoxia, noradrenaline (NA) content in the carotid body (CB) increases, suggesting that NA plays an important role in CB chemotransduction. However, it is unknown whether short-term hypoxia upregulates NA biosynthesis in CB. Therefore, we examined dopamine β-hydroxylase (DBH) expression in the CB of rats exposed to hypoxia (10% O2) for 0 to 24 hr with immunoblotting and immu…

Phloroglucinol Protects Small Intestines of Mice from Ionizing Radiation by R…

Phloroglucinol (PG) is a phenolic compound isolated from Ecklonia cava, a brown algae abundant on Jeju island, Korea. Previous reports have suggested that PG exerts antioxidative and cytoprotective effects against oxidative stress. In this study, we confirmed that PG protected against small intestinal damage caused by ionizing radiation, and we investigated its protective mechanism in detail…

Identification of the Protein Target of Myelin-Binding Ligands by Immunohisto…

The ability to visualize myelin is important in the diagnosis of demyelinating disorders and the detection of myelin-containing nerves during surgery. The development of myelin-selective imaging agents requires that a defined target for these agents be identified and that a robust assay against the target be developed to allow for assessment of structure-activity relationships. We describe an i…

Prior Experience with Photostimulation Enhances Photo-Induced Reproductive Re…

In vertebrates, reproductive output often increases with age. Unlike older birds, first-year photoperiodic birds lack experience with the reproductively stimulatory effects of long day lengths (photostimulation). We examined whether age-related differences in annual reproductive development could be partially attributed to previous experience with photostimulation in the photoperiodic house fin…

Role of macrophage activation in the lipid metabolism of postprandial triacyl…

The potential link between the inflammatory effects of postprandial lipemia and the induction of macrophage foam cell formation by triacylglycerol-rich lipoproteins (TGRL) was studied using postprandial triacylglycerol-rich lipoproteins (ppTGRL) derived from human volunteers and primary human monocyte-derived macrophages (HMDM). Subjects were fed a test meal high in dairy fat, followed three…

Delaying embryo development by storing at 48C for synchronization to recipien…

Short-term storage of embryos at low temperature induces developmental arrest of the embryo and would appear to be a valuable aid in embryo-transfer techniques to avoid wasting embryos. Embryo storage at 48C was examined to allow synchronization with embryo-transfer recipients using the microinjection technique. Superovulation was induced in female Japanese White donor rabbits four days befo…

Between-assay variability of faecal calprotectin enzyme-linked immunosorbent …

Faecal calprotectin (f-Cp), a marker of intestinal inflammation, can be used to distinguish between functional and organic bowel disease. F-Cp, following extraction, is commonly quantified using enzyme-linked immunosorbent assays (ELISAs) but there are no data comparing the different f-Cp assays or sample extraction devices. We, therefore, evaluated and compared the performance of the Immunod…

Relationship of total 25-OH vitamin D concentrations to Indices of Multiple D…

Vitamin D deficiency appears to be widespread and associated with ethnicity and economic status. Geography is the key to virtually all national statistics. It provides a structure for collecting, processing, storing and aggregating data.Linking geographic data to laboratory data allows analysis of the association of laboratory data with economic indicators.Methods: The laboratory information s…

Interference of ethylene glycol with L-lactate measurement is assay-dependent

Background: Metabolites of ethylene glycol (EG) can cross-react in L-lactate assays, leading to falsely elevated serum lactate levels in case of EG intoxication. In this study, we evaluated the effects of EG and its metabolites on routinely used lactate measuring methods. Methods: Serum aliquots were spiked with either L-lactate, EG or one of its metabolites (all 12.5 mmol/L): glyoxal, glycolat…

Impact of prandial status on the comparison of capillary glucose meter and ve…

Background: There is a negative glucose gradient between the capillary and venous systems, produced by glucose uptake into peripheral tissues. This gradient is augmented by oral glucose ingestion in healthy volunteers; thus prandial status may impact on capillary glucose meter performance. Our primary aim was to investigate whether the (capillary–venous plasma) glucose difference changed in r…

Identification of a rare variant haemoglobin (Hb Sinai-Baltimore)causing spur…

Commonly used methods for assay of haemoglobin A1c (HbA1c) are susceptible to interference from the presence of haemoglobin variants. In many systems, the common variants can be identified but scientists and pathologists must remain vigilant for more subtle variants that may result in spuriously high or low HbA1c values. It is clearly important to recognize these events whether HbA1c is being u…

Iron autointoxication in a 16-year-old girl: a protective role for hepcidin?

Intentional iron overdose appears to be an increasingly common form of attempted suicide. We present a case of iron overdose in a 16-year-old girl who was found unconscious in her bed and brought to our emergency department. The most remarkable diagnostic findings were the patient’s comatose condition, divergent eye position and positive Babinski foot pad reflexes. Laboratory tests showed hyp…

Consideration of the degree of increase in urine metadrenalines provides supe…

Background: Measurement of fractionated plasma or urine metadrenalines is the recommended screening test in the diagnosis of phaeochromocytoma, with clinical cut-offs geared towards diagnostic sensitivity. Current practice at Salford Royal Hospital is to add urine catecholamines onto samples with raised urine metadrenalines, with the aim of adding specificity to a diagnosis of phaeochromocytoma…

Pioglitazone protects HDL2&3 against oxidation in overweight and obese men

Background: The worldwide epidemic of obesity is a major public health concern and is persuasively linked to the rising prevalence of diabetes and cardiovascular disease. Obesity is often associated with an abnormal lipoprotein profile, which may be partly negated by pioglitazone intervention, as this can influence the composition and oxidation characteristics of low-density lipoprotein (LDL). …

‘Sweet Dreams’, ‘Happy Days’ and elevated 24-h urine 5-hydroxyindolea…

We report two patients with markedly elevated 24-h urine 5-hydroxyindoleacetic acid (5-HIAA) excretion due to over-thecounter (OTC) self-medication with 5-hydroxytryptophan (5-HTP). It is important to recognize that OTC medication may cause increased ‘false-positive’ 5-HIAA excretion to prevent undue patient anxiety and unnecessary further investigation for carcinoid disease. Discordance be…

Sodium fluoride: an ineffective inhibitor of glycolysis

Entrenched in current laboratory protocols for the measurement of plasma glucose is the false belief that sodium fluoride (NaF) is an effective inhibitor of glycolysis. The failure of NaF to properly control glycolysis decreases plasma glucose concentrations. The amount of the decrease can be clinically significant. The most recent (2011) laboratory guidelines for diagnosis and management of di…

Vitamin A supplementation, serum lipids, liver enzymes and C-reactive protein…

Background: High-dose vitamin A influences glucose and lipid profile; however, the possible effects of moderate doses (25,000 IU/d) are conflicting. We aimed to compare the effect of vitamin A pplementation on several anthropometric and biochemical variables between obese and non-obese women. Methods: This study was performed on 84 women among whom 56 were obese (body mass index [BMI] 30–35 …

Reference intervals for plasma free metanephrines with an age adjustment for …

Background: Measurements of plasma normetanephrine and metanephrine provide a useful diagnostic test for phaeochromocytoma,but this depends on appropriate reference intervals. Upper cut-offs set too high compromise diagnostic sensitivity,whereas set too low, false-positives are a problem. This study aimed to establish optimal reference intervals forplasma normetanephrine and metanephrine. Metho…

A new accurate, simple formula for LDL-cholesterol estimation based on direct…

Background: Low-density lipoprotein cholesterol (LDL-c) is the major measured parameter for cardiovascular risk assessment. The generally accepted formula (LDL-F) for estimating LDL-c developed by Friedewald and colleagues in 1972 using data from 448 individuals suffers from known inaccuracies at extremes of triglyceride (TG) and total cholesterol (TC) values. Methods: We generated new formulas…

The relative effects of fat versus muscle mass on cystatin C and estimates of…

Background: It is well known that plasma creatinine concentration is affected by muscle mass, while some studies have suggested cystatin C is affected by body mass index (BMI). Our aim was to assess the effects of lean versus fat mass on cystatin C and creatinine derivative equations in estimating glomerular filtration rate (GFR) in healthy young men. Methods: Three groups of participants were …

Which point-of-care creatinine analyser for radiology: direct comparison of t…

Availability of whole blood creatinine estimation for patients scheduled to undergo radiological contrast investigations can provide information to aid patient care by reducing adverse effects and improving departmental efficiencies. Methods: We performed imprecision studies, different patient sample type comparison in 40 participants, and a limited interference study with dopamine and dobutami…

Biochemistry of Plant Secondary Metabolism

A characteristic feature of plants is their capacity to synthesize and store a wide variety of low molecular weight compounds, the so-called secondary metabolites (SMs) or natural products. The number of described structures exceeds 100 000; the real number in nature is certainly much higher because only 20–30% of plants have been investigated in phytochemistry so far. In contrast to pri…

TiO2-doped phosphate glass microcarriers: A stable bioactive substrate for ex…

Scalable expansion of cells for regenerative cell therapy or to produce large quantities for high-throughput screening remains a challenge for bioprocess engineers. Laboratory scale cell expansion using t-flasks requires frequent passaging that exposes cells to many poorly defined bioprocess forces that can cause damage or alter their phenotype. Microcarriers offer a potential solution to scala…

Thermosensitive poly(Nisopropyl acrylamide-co-N,Ndimethyl acryl amide)-blockp…

Novel amphiphilic block copolymers consisting of hydrophobic poly(d,l-lactide) segments and hydrophilic poly(N-isopropylacrylamide-co-N, N-dimethylacrylamide) blocks were designed and synthesized through a simple free radical copolymerization route based on a bifunctional initiator, followed by the ring-opening polymerization of d,l-lactide. The copolymers self-assembled into thermosensitiv…

Thermal and mechanical properties of POSS-Cyanate ester/epoxy nanocomposites

A new cyanate ester functionalized polyhedral oligomeric silsesquioxane (POSS-Cy) monomer was prepared by reacting hydroxyl functionalized POSS-OH with cyanogen bromide (CNBr) in the presence of triethylamine (Et3N). The precursor POSS-OH and the corresponding cyanate ester POSS-Cy were characterized by Fourier transform infrared (FTIR), 1H and 13C nuclear magnetic resonance (13C-NMR) spectral …

The influence of nanosilica functionalization on the properties of hybrid nan…

The aim of this research is to evaluate the effect of silica nanoparticles (SNs) modification on the physicochemical properties of some hybrid nanocomposites. SNs with high specific surface area were silanized by two complementary mechanisms using organosilanes with different functionalities, 3-methacryloxypropyltrimethoxysilane (MPS),octyltrimethoxysilane (OTMS) or a mixture of them in order t…

A Meta-analysis Comparing Lightweight Meshes With Heavyweight Meshes in Licht…

Background. To evaluate the influence of lightweight and heavyweight mesh on postoperative recovery in Lichtenstein inguinal hernia repair. Methods. PubMed, EMBASE, and the Cochrane library were used to search for published clinical randomized controlled trials (RCTs), which compared lightweight meshes with heavyweight meshes in Lichtenstein inguinal hernia repair. Two independent reviewers ass…

Laparoscopic Splenectomy and Esophagogastric Devascularization Is a Safe, Eff…

Open splenectomy and esophagogastric devascularization (OSED) is a typical surgery for portal hypertension. Because of the high morbidity associated with it, it is desirable to develop a minimally invasive alternative. To investigate the safety and effect of laparoscopic splenectomy and esophagogastric devascularization (LSED), we performed LSED for 24 patients suffering from portal hypertensio…

Is Single-Port Access Laparoscopy Less Painful Than Conventional Laparoscopy …

Objective. This study aimed to compare postoperative pain and surgical outcomes after transumbilical single-port access (SPA) and conventional multiport laparoscopic surgery for adnexal lesions. Methods. A retrospective case–control study was conducted matched by age, body mass index, and frequency of previous abdominal surgery. A total of 110 SPA laparoscopy patients (cases) were matched wit…

Multimodal Imaging and Detection Strategy With 124 I-Labeled Chimeric Monoclo…

Renal cell carcinoma (RCC) accounts for approximately 85% to 90% of all primary kidney malignancies, with clear cell RCC (ccRCC) constituting approximately 70% to 85% of all RCCs. This study describes an innovative multimodal imaging and detection strategy that uses 124I-labeled chimeric monoclonal antibody G250 (124I-cG250) for accurate preoperative and intraoperative localization and onfirmat…

The Use of Multimedia Consent Programs for Surgical Procedures: A Systematic …

Objective. To compare multimedia and standard consent, in respect to patient comprehension, anxiety, and satisfaction, for various surgical/interventional procedures. Data sources. Electronic searches of PubMed, MEDLINE, Ovid, Embase, and Google Scholar were performed. Relevant articles were assessed by 2 independent reviewers. Study selection. Comparative (randomized and nonrandomized control …

Five Really Easy Steps to Build a Homemade Low-Cost Simulator

Aim. The aim of this study was to evaluate how simple it is to build a homemade low-cost simulator using a simple 5-step scheme. Methods. A scheme explaining how to build an endoscopic surgery simulator in 5 easy steps was presented to 26 surgeons. The simulator required a pair of scissors and easy-to-find materials. Its total cost was less than €35. The participants assessed the simulator us…

Single-Incision Laparoscopic Splenectomy Versus Conventional Multiport Laparo…

Background. The authors report their experience with single-incision laparoscopic splenectomy (SLS) and compare postoperative outcomes of conventional multiport laparoscopic splenectomy (MLS) with SLS in patients with spleen sizes ≤15cm. Methods. Demographic, intraoperative, and postoperative data were analyzed retrospectively and compared between patients who underwent MLS from June 20, 2006…

Radiofrequency-Assisted Partial Nephrectomy for Metanephric Adenoma: A Case R…

Metanephric adenoma (MA) is a rare benign tumor, diagnosis of which is often carried out after surgical treatment. In case of peripheral lesions, a partial nephrectomy (PN)—either open or laparoscopic may be preferred—and, furthermore, a radiofrequency (RF)-assisted procedure may facilitate adequate hemostasis. In November 2010, the authors performed a RF-assisted PN, according to Habib’s…

An Ear-Worn Sensor for the Detection of Gait Impairment After Abdominal Surgery

Surgery to the trunk often results in a change of gait, most pronounced during walking. This change is usually transient, often as a result of wound pain, and returns to normal as the patient recovers. Quantifying and monitoring gait impairment therefore represents a novel means of functional postoperative home recovery follow-up. Until now, this type of assessment could only be made in a gait …

A Rigid Disc for Protection of Exposed Blood Vessels During Negative Pressure…

Background. There are increasing reports of serious complications and deaths associated with negative pressure wound therapy (NPWT). Bleeding may occur when NPWT is applied to a wound with exposed blood vessels. Inserting a rigid disc in the wound may protect these structures. The authors examined the effects of rigid discs on wound bed tissue pressure and blood flow through a large blood vesse…

The Use of the Harmonic Scalpel Versus Knot Tying for Modified Radical Neck D…

Background. Although the utility of the harmonic scalpel (HS) in thyroidectomy has been extensively demonstrated,there is little experience regarding its use for neck dissections. Methods. Within 10 years, 119 patients underwent modified radical neck dissection (MRND) for thyroid cancer. In 51 patients, MRND was performed using conventional knot tying and in 68 using the HS. The number of lymph…

Study on the nonisothermal crystallization kinetics of ternary-monomer solid-…

In order to discover how the grafted side chains affect the crystallization of the graft copolymers, the ternary-monomer solid-phase graft copolymers of polypropylene (PP; PPSM) were synthesized by solid-phase grafting of maleic anhydride, styrene and butyl acrylate onto PP. The Jeziorny and Mo methods were used to investigate the nonisothermal crystallization kinetics and polarizing microscop…